FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2248_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2248_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704077
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG40630.23842819309221355No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT40630.23842819309221355No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA40350.23678507485283823No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC40180.23578746735036035No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA32720.1920101028298604No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG32370.18995620503064123No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT24450.14347943197402466No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19840.11642666381859504No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18850.11061706718651798No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT18130.10639190599955282No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA17850.1047487877601775No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG17630.10345776628638259No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG17430.1022841104011145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC451.2314049E-680.575951
AGACCGC1750.066.28499145
GTTACGT1950.055.7833481
ATGCCGG1950.055.76862145
GACAGTA9950.054.6476447
GACGACT1353.6379788E-1253.71734
AGCGAAA3450.052.5495381
GGTACTG9950.051.017945
GCAGGTA10400.049.5077252
GTATGGA16700.049.069311
GTAGAAA15350.048.6605262
CGATTAT450.00964070948.332806145
GTACTGA10800.047.002646
TGGATAG17600.046.560084
TTACGTG2350.046.2883152
ATAGCAA17700.046.2848057
TTGGACA11500.046.033394
ACAGTAT11500.046.0212368
ACTGATC12200.045.7576948
CAGTATG11800.045.4656079
Produced by FastQC (version 0.11.5)