FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419591
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC78430.5524830743502882No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC75290.5303640273853525No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT69630.490493388588685No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG69030.4862668191049394No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT66090.4655566286345856No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA63940.4504114213178303No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT62680.44153562540196434No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC62520.4404085402062988No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA60030.4228682768487544No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC57180.4027920718009624No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT55260.38926704945297624No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG54320.38264542392844136No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT53070.37384007083730453No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG45510.3205852953421091No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG44320.31220259919934684No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA42580.2999455476964844No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG42540.299663776397568No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA39960.28148952761746165No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA38850.27367037407253214No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT38370.2702891184855356No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG38280.2696551330629738No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA37030.26084977997183695No Hit
GATAAACAGAATTTGCCTGGCGGCCTTAGCGCGGTGGTCCCACCTGACCC35730.25169221275705467No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC35200.24795874304641266No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG34690.24436615898522884No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG33750.23774453346069396No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA33580.23654700544029936No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT32750.23070025098778452No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG32700.23034803686413904No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA32700.23034803686413904No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT32210.22689633845241342No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC31690.2232333115665005No Hit
GATTAAATCAGAACGCAGAAGCGGTCTGATAAACAGAATTTGCCTGGCGG30620.2156959293204874No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG30580.21541415802157102No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG29900.21062404593999257No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA28830.20308666369397949No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT28670.20195957849831395No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG27970.19702858076727733No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC27770.19561972427269547No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG27710.19519706732432088No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA27680.1949857388501336No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG27200.19160448326313706No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG27190.19153404043840797No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA27020.19033651241801336No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT26990.19012518394382608No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA26180.18441931514076942No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT26180.18441931514076942No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT26100.18385577254293664No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA25160.1772341470184018No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT24800.17469820532815436No Hit
GCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATC24580.17314846318411428No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG24350.1715282782153451No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC24260.17089429279278326No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG24150.17011942172076325No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA24090.16969676477238865No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT23840.1679356941541613No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC23330.16434311009297747No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT23090.1626524822994792No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT22680.15976432648558633No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT22600.15920078388775358No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT21960.15469244310509153No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC21790.15349491508469693No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC21260.14976144537405492No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG20990.14785948910636937No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG20900.14722550368380752No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA20850.14687328956016205No Hit
AACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTT20440.14398513374626917No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC20340.14328070549897823No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC20320.14313981984952004No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCAC20260.14271716290114547No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC20180.14215362030331272No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT20140.14187184900439634No Hit
CTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTG20000.14088564945818902No Hit
GGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTT19850.1398290070872526No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA19830.1396881214377944No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA19450.13701129409808882No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT19370.13644775150025606No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG19320.1360955373766106No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT19190.13517978065513236No Hit
GTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCT19160.13496845218094508No Hit
CCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCG19150.134898009356216No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT19080.13440490958311233No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT18730.13193941071759402No Hit
CTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAG18220.1283468266564102No Hit
GGTTAAGGATAAGCACCTTTAGCGGACGAATTTCCTGACCAGACGCGCGA17970.1265857560381828No Hit
CCCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGC17830.12559955649197552No Hit
TATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCG17680.1245429141210391No Hit
GTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGA17530.12348627175010267No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC17440.1228522863275408No Hit
GTGCCATTGGCATGACAACCCGAACACCAGTGATGCGTCCACTCCGGTCC17420.12271140067808264No Hit
CTACCGGGCAGTGCCATTGGCATGACAACCCGAACACCAGTGATGCGTCC17310.12193652960606259No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG17190.12109121570931344No Hit
TTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCGAACACCAGTGATG16950.11940058791581518No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC16920.11918925944162789No Hit
CCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTC16880.11890748814271154No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG16840.11862571684379515No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG16620.11707597469975507No Hit
CCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTG16190.114046933236404No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA16130.11362427628802943No Hit
ACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTA16080.11327206216438397No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGCTC16040.1129902908654676No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA15850.1116518771956148No Hit
GTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCT15840.1115814343708857No Hit
GCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAG15770.11108833459778204No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC15710.11066567764940746No Hit
GCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGC15620.11003169222684561No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA15430.10869327855699282No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT15350.10812973595916006No Hit
ATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGC15210.10714353641295274No Hit
GGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCG14910.1050302516710799No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT14900.10495980884635081No Hit
GTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAA14840.10453715189797624No Hit
AATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATG14720.10369183800122711No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT14660.10326918105285254No Hit
GTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTC14450.10178988173354156No Hit
CCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTG14450.10178988173354156No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG14270.10052191088841787No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC14240.10031058241423059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC23650.067.775883
GTCATAG11400.067.4396062
CATAGTG11500.066.853184
ACGCGCC40400.065.858998
CAAACGC16650.065.7774055
TAAGACG24300.064.769044
CGCGCCA41100.064.560919
AAGACGC25650.061.642915
TCATAGT13000.059.1393473
GATAAGA27800.057.919152
ATAGTGA14550.053.836255
AGACGCG29900.053.123536
GACGCGC29650.053.0597847
AACGCGC20850.052.5050777
GGAGCAA21300.051.758061
AGTGATC15650.049.567827
AAACGCG22450.049.106766
GACGGAT6500.049.0760657
GGATAAG35600.048.8962171
TAGTGAT16150.048.5026256
Produced by FastQC (version 0.11.5)