FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064035
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC4150.050.6623957
CGCGCCA4550.049.395369
ACGCGCC5700.045.7892468
CGCGTAT1401.41590135E-841.428364145
TCTCCGC8150.036.47221145
ACCGATA1209.569116E-636.249817145
GCGAAAG7500.032.8704832
TGATCCG3750.028.9998559
AGCGAAA8700.028.3366221
AAGACGC6800.027.7238085
CGAAAGC9350.027.142193
AGACGCG6500.026.7723396
CACGAGC8000.025.37487145
AAACGCG2301.1029515E-625.220326
TATACTG12650.025.220325
ATAAGCG1500.001493800124.1694743
ATACCGT1500.001493800124.1694746
GCACGGT1550.001807916823.3898126
ATAAGAC10450.022.8973963
GTATAAG6750.022.5581741
Produced by FastQC (version 0.11.5)