FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777390
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18300.10295995814086949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG1001.390814E-436.254741
GAATAAC7550.027.8513261
AGCGAAA8350.027.7880651
TCTCCGC8300.027.078049145
GTCCTAT12850.025.9567011
GCGAAAG8800.025.5431142
GACGCGC1500.001494570624.1671097
GGATAGC9350.024.0405775
GTATGGA12750.022.179371
CGAAAGC10150.022.1457523
TATAATA6900.022.0681022
GTGATCG2104.3154202E-420.7146639
GGTACTG8450.020.5944085
CACGAGC5650.020.530771145
TCCTATG15550.020.517152
TATACTG8150.020.46285
TAATACG1850.00504457519.5971574
GTATTAG6000.019.3358611
TGGATAG11800.019.04914
TAGGACT13150.018.74774
Produced by FastQC (version 0.11.5)