FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307136
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC27380.11867527531970375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC5400.051.0213978
AACGCGC2850.048.3350147
CGCGCCA6450.042.715599
GACGCGC3800.036.2512637
GACTCGC3150.034.523514145
AAACGCG4350.031.67126
CGAAAGC9250.028.2196463
GCGAAAG9150.027.735612
TTATTCG1907.1612158E-626.710299145
GTATTAG8150.026.6903611
AGCGAAA9950.026.2343441
GTATATA11300.024.3834931
GAATAAC14450.024.0859721
AGACGCG5950.023.1545756
GTATAAG6700.021.6444231
CAAACGC6800.021.326125
TGATCCG4451.1459633E-1021.1809739
ATAAGAC13100.020.4795863
GCAAACG6850.020.1119334
TCGACGG5505.456968E-1219.77256145
Produced by FastQC (version 0.11.5)