FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748293
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18020.1030719679138451No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA3100.051.4525269
AACGCGC1453.8562575E-1045.00087
GCGAAAG5650.042.3543662
GACGCGC2450.041.429317
ACGCGCC3850.041.4293068
AGCGAAA6100.038.041081
CGAAAGC6500.035.700093
TCTCCGC6200.029.233553145
AAACGCG2003.249679E-729.0071546
AGACGCG3600.028.2013976
GTATACG2354.2924512E-827.7720091
TCGCGTT1751.2563437E-424.856873145
GCACCGT6150.024.7622036
GTGATCG2651.3773206E-724.623089
TATACTG10000.023.9302165
ATCGCGG2751.970584E-723.727016145
ATAGACG1650.002598340421.9744873
GTATTAT20550.021.8782461
ATACCGC4006.89397E-1021.7553656
GCGTTAA2353.7107173E-521.5961289
Produced by FastQC (version 0.11.5)