FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361875
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC800.00203652236.2512742
AGCGAAA3650.035.7546841
GCGAAAG4750.027.4746512
GTTACGT1409.994401E-425.8937681
CGAAAGC5050.025.8424953
GTTGACA9000.025.7786851
AGACGGT2802.3439497E-723.303535145
GCCTATA3203.3865945E-822.6570471
TATACTG5800.022.5007935
GTAACGG2903.2867501E-722.5007931
GTGATCG1700.003093390321.3234969
ATGCCGG1700.003093390321.323496145
ACGGGTT2404.3631902E-521.1465784
AACGGGT2404.3631902E-521.1465783
TACACTG7550.021.1265725
TAGGTAG6950.020.8640425
ACATGGT11250.020.6229485
TTAGGTA6400.020.3913424
GCACCGT8950.020.252116
TGTACCG2155.0562277E-420.2332695
Produced by FastQC (version 0.11.5)