FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2246_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2246_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489484
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1453.8562575E-1045.000947
AACGCGC750.001483467938.6674737
CGCGCCA2250.038.667479
ACGCGCC2250.038.667478
GCGTTAA2301.0040822E-931.5223949
AGTCGAA1851.6408558E-731.3593732
AGCGAAA4450.030.9629781
GCGAAAG4650.029.6312372
TGCGTTA2654.5602064E-927.359068
TACTGCG2756.7520887E-926.3703825
AAGTCGA2207.473391E-726.3703821
CGAAAGC5250.026.244813
GTCGAAG2401.595401E-624.1728523
GTATACG2451.9088766E-623.6795271
TGATCCG2151.8662315E-523.6051459
GTTACGT2603.1988002E-622.31341
GTTGACA12850.022.0095211
CTACTGA17650.021.365246
GTATTAT17100.021.2042561
AGACGCG2755.2019514E-621.0963066
Produced by FastQC (version 0.11.5)