FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801125
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6300.042.5884322
AGCGAAA6450.041.5980031
CGAAAGC7200.036.2577173
TATATCG850.002739704434.1249125
GACCGGC3850.033.895794145
GGACCGT2354.292815E-827.771876
GTGATCG1450.001226890824.9997719
CACGAGC6000.024.166445145
GTTACGT3652.1827873E-1023.8406931
TGCACCG1902.1909548E-422.8996125
CCGACTA4557.2759576E-1222.3124434
GTAGTAC2806.083512E-620.7186973
ATGCCGG3301.1391166E-619.772545145
GTATATA12350.019.3765951
TTACGTG4904.129106E-1019.238792
CCGTTCA2659.355636E-519.1507689
GAGCGAA2359.1908185E-418.514581
TATACTG10800.018.1288595
GTATAAG6451.8189894E-1217.9883271
TCTCCGC7700.017.889446145
Produced by FastQC (version 0.11.5)