FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231334
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC8450.036.892826145
CTCGTAA800.002034542236.2581831
AGCGAAA4100.030.0677641
TCTCCGC7750.028.999481145
GCGAAAG4300.028.669262
CTACGCA1804.6899077E-628.20314
CCCACGC7850.027.70651145
CGTAAAC1409.97802E-425.9008063
TCTACGC1751.2533406E-424.8647753
CCACGAC3601.8553692E-1024.166233145
GTGACCG2852.7760143E-722.8980469
CGAAAGC5800.021.2565253
GTTAGAC3457.483686E-821.0209453
TAGTACC5605.456968E-1219.4256044
CTCTACG2256.8584847E-419.3376982
GGTCGGT2359.19279E-418.5133158
TACTCCG2801.4230711E-418.1305644
GTGCTAG4455.527727E-817.9253941
TACGCAA2450.001214638617.7605535
GTCTAAG6152.5465852E-1117.6869181
Produced by FastQC (version 0.11.5)