FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908074
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2850.038.1654661
CGAAGCG850.002742405334.1181569
GACGACT2008.942152E-932.6314774
TTACGTG3650.029.8004362
TTACCCG1409.986357E-425.8979955
TATACGT2601.1436896E-725.1011334
GCGAAAG5650.024.385372
TAGCCCG1500.001493043124.1714615
GTTATGT13250.023.5329721
AGTCGAA1550.001807000723.3917392
TACGTGT4700.023.142893
AGCGAAA6150.022.4028191
GTATACG1650.002598721321.9740583
ACCGATC1650.002601490821.9700268
CGAAAGC6350.021.697223
TAGTACC12550.021.3787424
GCACCGT7050.020.5719996
TACACCG2505.969016E-520.3040315
ATCTATG8700.020.003971
CCGACTA4351.9608706E-920.003974
Produced by FastQC (version 0.11.5)