FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658737
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18710.11279666396782613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2353.45608E-1133.945881
CGAGGCG3650.033.7666145
CGTATAC2051.1361408E-831.8393363
GCGAAAG5100.029.8623872
TTACGTG2701.70985E-1029.546382
AGACCGC3201.8189894E-1229.452671145
AGCGAAA5200.029.2872221
CACGAGC5600.028.481703145
ATGCCGG2954.838512E-1027.033482145
CGAAAGC5700.026.7189753
GCTACTA4150.026.2052069
TAGTACC10100.025.8419679
GTTATGT11100.025.4802631
GTTGACA15150.025.3702931
CCGACTA4201.8189894E-1224.1743094
ACATGGT16600.024.029415
TCTCCGC7550.024.006254145
TACGGGG1952.6149562E-422.3086937
TACGCAG1700.003087433321.3309175
GTGATCG1700.003093378421.3238459
Produced by FastQC (version 0.11.5)