FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d08.2_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d08.2_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659227
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10270.15578852201138607No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC9870.14972080937218896No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT6940.10527481429007005No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG6880.10436465739419047No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT6800.10315111486635105No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA6750.1023926507864514No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC6620.10042064417871235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC607.2759576E-1296.6666647
ACGCGCC1450.070.08
CGCGCCA1700.059.705889
AACGCGC753.7592326E-758.07
AAACGCG951.9198815E-645.7894746
ACATGGT7850.037.866245
GTTACGT1354.967951E-737.5925941
GTTGACA7800.037.1794851
GGGTAAC1458.692441E-735.01
AAGACGC1751.0083022E-733.1428575
GGGTTAC900.00362734232.222221
AGACGCG1851.6421836E-731.3513536
GCATGTT9500.031.2894744
TGACATG10000.031.1753
TCCGTAC950.00472613930.5263183
CGGTCCA1902.0747757E-730.5263189
TAAGACG1902.0747757E-730.5263184
GTAATAT2151.8084393E-830.3488371
TAGCATG9650.030.0518112
TATACTG3151.8189894E-1229.9206335
Produced by FastQC (version 0.11.5)