FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2244_d08.2_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2244_d08.2_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823071
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18800.1031226979091873No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18300.10038007296479401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1600.054.3742527
GCGAAAG5300.041.0495572
CGCGCCA2850.040.702319
ACGCGCC3100.039.7575158
AGCGAAA5800.037.51081
CGAAAGC5950.036.5651473
AACGCGC1001.3920118E-436.24957
CACGAGC6000.027.79128145
TATACTG9350.027.1468525
TCTCCGC6150.027.113447145
GTTACGT3108.56744E-1025.7332151
AAACGCG1450.001224773525.0071986
TGATCCG1751.2561641E-424.8574819
CTCCGAC2101.5561474E-524.166332145
ATAAGAC8150.024.0253243
CCGACTA4251.8189894E-1223.889234
AGACGCG3702.6011548E-1023.5202876
ATGCCGG3753.092282E-1023.19968145
GCGTTAA3152.8680006E-823.0161869
TTACGTG3604.838512E-922.1591572
Produced by FastQC (version 0.11.5)