FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2243_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2243_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473173
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC349192.3703258205248128No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG339592.3051603579484556No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG222651.5113635669402032No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA98970.6718151907481335No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA81070.5503087553193006No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG80310.545149822865339No Hit
GAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA78990.5361895717610898No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT78570.5333385827733742No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTATATTC66530.4516102317921928No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT62070.4213354439702601No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC61490.41739836393960517No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA57860.39275767340292006No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG57270.38875271268208145No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAA54140.36750605665458164No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA54030.36675936906256085No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT53770.36499447111778455No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA53490.3630938117926408No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA50660.3438835764706521No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG49110.3333620694921777No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT48820.3313935294768503No Hit
CCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAAC47900.32514850597994943No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG46820.3178173914401092No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC45950.31191177139412685No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG44370.30118662234510135No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA41700.28306247806605195No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA41410.2810939380507245No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA41390.2809581766703571No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC40450.2745773917930888No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA40010.2715906414250058No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT38320.2601188047839595No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG37700.2559102019925698No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG37130.25204100265209856No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT37070.25163371851099636No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA36910.25054762746805703No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA36300.246406905366851No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA35160.23866850668590858No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT33990.23072646593441504No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA33460.22712878935467865No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC32490.2205443624068592No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG32060.21762549272895987No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA31560.2142314582197746No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG31560.2142314582197746No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG31360.21287384441610047No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT31090.21104106578114043No Hit
CTCTCTGGGTCCTCTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAG31020.2105659009498545No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30530.2072397471308529No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA30230.20520332642534178No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG29690.20153776915542168No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT29410.19963710983027788No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG29350.1992298256891757No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT28320.192238114600254No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT26850.1822596531432493No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG26610.18063051657884036No Hit
CAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAA26300.1785262151831455No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA25150.17071993581201936No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC24630.16719013992246667No Hit
CCTTCCCGATTGAGCCTTCCTCCACTCCCTTAGGTGACTCTCCAATTGGC24570.16678285578136443No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG24260.16467855438566958No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT24180.16413550886419992No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT23850.16189544608813763No Hit
CCCATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAGA23790.1614881619470354No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA23730.1610808778059332No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG23530.15972326400225909No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA23490.15945174124152425No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC23470.15931597986115684No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC23360.15856929226913608No Hit
GGGTACTCCCCTGCATTTAACTACAACAAGACAACTAAGAGGCTTACAAT23080.1566686329439923No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC22800.1547679736188486No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACT22570.15320671774462336No Hit
AGTCTGCTTCATGTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA21370.14506103492257866No Hit
GTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCT21160.14363554042872087No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA21050.1428888528367001No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT20920.14200640386431193No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCA19750.13406436311281839No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG19700.13372495966189984No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTAC19290.13094185136436792No Hit
GTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACTACT18990.12890543065885676No Hit
GTTCATTGATGCTTAATGCTGGTCTTTTGTCTTCTTTGCCGAGGATTAGA18980.12883754996867305No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT18570.12605444167114113No Hit
CTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATG18440.12517199269875295No Hit
CATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAATGGATGATG18000.12218524233066992No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC17950.1218458388797514No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG17580.11933425334295429No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG17150.11641538366505494No Hit
CCCAGAGAGTCTGCAGTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGA16990.11532929262211566No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA16960.11512565055156454No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA16960.11512565055156454No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG16720.11349651398715561No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC16600.11268194570495116No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG16570.11247830363440003No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG16560.11241042294421633No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG16490.11193525811293038No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA16200.10996671809760292No Hit
ATTGTGGAATCTGGTGATCCAAATGCATTGTTGAAGCACAGGTTTGAAAT16030.10881274636447993No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15850.10759089394117324No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGC15680.10643692220805023No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT15600.10589387668658061No Hit
CTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAGCCTCTTAGTTGTC15590.1058259959963969No Hit
GGATACACCGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATG15430.1047399049534576No Hit
CGCCTCGTTCGTCAATGAAATGGAAGTCTGAATACATGAAGCAGACTTCT15280.10372169460070202No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA15200.10317864907923237No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC15020.10195679665592568No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14980.10168527389519084No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA14950.10148163182463973No Hit
CTCTGGGTCCTCTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAGCC14890.10107434768353751No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCTTTTGTCTTCT14870.1009385863031701No Hit
CCCAGATGAAGGAACAGCAGGAGTAGAGTCTGCAGTATTGAGAGGATTTC14860.10087070561298639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA49000.0113.665762
AGCAGGT50500.0109.858721
GGTACTG51350.0108.605145
CAGGTAC51950.0107.21123
AGCGAAA4700.0104.924171
GTACTGA54350.0102.610386
ACTGATC55150.0101.883248
TACTGAT57900.096.668577
ACGCGTT259.388167E-486.99876145
GCGAAAG5650.084.7149352
AGGTACT70900.080.397174
CGAAAGC6350.075.376283
GTTACGT809.822543E-1172.521121
TTACGTG809.822543E-1172.521122
TTGGACA47300.066.081614
GACAGTA47300.065.75717
TTTTTGG47450.064.802861
CTGATCC87700.063.9037679
ACAGTAT49700.062.873478
TTTGGAC49750.062.827343
Produced by FastQC (version 0.11.5)