FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2243_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2243_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715853
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG372632.1716895328445966No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC334681.9505167400703909No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG257521.5008278681215699No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA131950.7690052702649935No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG100710.5869383915755021No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA84400.49188362872577085No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG80510.46921268896577967No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC74820.43605134006234797No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT71640.41751828390893625No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA71470.4165275230453891No Hit
GAATCGAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA70140.4087762762894024No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT70010.40801863562904284No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA67440.39304066257424153No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT66510.3876206178501305No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG64700.377071928655893No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT63800.3718267240841727No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC63010.3672226000712182No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG62370.36349267682021713No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA58150.3388984953839286No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA57860.3372083739108187No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA54260.3162275556239375No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT52370.30521262602332483No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC51160.2981607398769009No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAA49110.28621332946353795No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG47160.27484871955814394No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG46530.27117707635793975No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA46260.26960351498642365No Hit
AAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTATATTC45480.2650576710242661No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA45200.2634258296019531No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA45200.2634258296019531No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG45120.26295958919557794No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT44830.26126946772246806No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG44450.25905482579218614No Hit
CCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAAC43900.2558494229983571No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA41380.24116285019754025No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA39010.22735047815867676No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG38770.22595175693955133No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG38070.22187215338376887No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG37400.2179673899803771No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA36980.21551962784690762No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG36750.21417918667857913No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT36330.21173142454510965No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA36230.2111486240371407No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC35910.20928366241164015No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT35780.20852602175128057No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG35560.20724386063374892No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC33960.1979190525062462No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC33210.19354804869647924No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACT32310.18830284412475895No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT31440.18323247970542933No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA30840.17973567665761578No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA30750.17921115620044376No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT30690.1788614758956624No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA29590.17245067030800423No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT29570.17233411020641046No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT29300.17076054883489436No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG29280.17064398873330058No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA29040.16924526751417515No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC29030.16918698746337826No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC27860.16236822152014188No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT27480.16015357958985996No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTAC27440.1599204593866724No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA27380.15957077908189105No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG27260.15887141847232836No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG26730.15578257578009305No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG26650.15531633537371792No Hit
GTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACTACT25830.15053737120837274No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA23420.1364918789663217No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA23300.135792518356759No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT23110.13468519739161805No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA22660.1320625951057579No Hit
AGTCTGCTTCATGTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGA22500.1311301142930076No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGC22490.13107183424221072No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA22330.13013935342946045No Hit
CAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAA22120.1289154723627257No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT21490.1252438291625215No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCA21340.1243696284005681No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT21050.12267950692745823No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG20760.12098938545434836No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA20490.11941582408283226No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATAGC20400.11889130362566024No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA20400.11889130362566024No Hit
GGGTACTCCCCTGCATTTAACTACAACAAGACAACTAAGAGGCTTACAAT20290.11825022306689442No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA20250.11801710286370684No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG19870.11580246093342494No Hit
CTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATG19700.11481170006987777No Hit
GTAGTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACA19600.11422889956190886No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG19550.11393749930792439No Hit
ACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAG18940.11038241620931397No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG18810.10962477554895436No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC18760.1093333752949699No Hit
CCCATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAGA18170.10589485229795326No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC18100.10548689194237501No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT17930.10449613107882784No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG17920.10443785102803096No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG17910.10437957097723406No Hit
GAGTAGTAGTTTTCTAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCT17840.10397161062165582No Hit
GTTCATTGATGCTTAATGCTGGTCTTTTGTCTTCTTTGCCGAGGATTAGA17720.1032722500120931No Hit
GTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAA17620.10268944950412419No Hit
ATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAAC17620.10268944950412419No Hit
GAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGA17580.10245632930093662No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG17520.10210664899615526No Hit
ATGTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACC17500.10199008889456149No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG17470.10181524874217081No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT17380.10129072828499877No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA17290.10076620782782675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA46600.0109.3845442
AGCAGGT47800.0106.790181
GGTACTG47800.0106.3351065
CAGGTAC49800.0102.50143
GTACTGA52050.097.65266
ACTGATC54400.093.8230748
TACTGAT57200.088.9768457
AGCGAAA4150.075.1288761
GTACGAC300.001935121172.50813
AGGTACT74350.070.801454
TTGGACA63250.064.884734
GACAGTA63400.064.7236567
TTTTTGG63550.064.350231
TTTGGAC65750.062.3073433
GTAGAAA35650.062.236972
TTGTACG350.0035653662.1498031
ACAGTAT66550.061.6601078
CTGATCC82950.060.6567279
AGTAGAA37150.059.5288541
TAGAAAC38600.057.668363
Produced by FastQC (version 0.11.5)