FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2243_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2243_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716721
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC186841.0883539025852191No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG137640.8017610316411344No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG85630.4987997467264628No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA71730.4178314356264064No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG56490.3290575463339704No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA55150.32125196814159085No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG54690.31857244129943074No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC47600.2772727775800494No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT44040.2565355698450709No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA40900.23824488661815169No Hit
GCCTAGAAAACTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAAC38780.225895762910805No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA38060.22170172089698909No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG37750.21989595280770724No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA36660.21354663920345823No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGCTGTTGAACAC34940.20352753883712027No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC34570.20137226724668716No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG31620.18418834510674711No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT30860.17976130075883034No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA28450.16572291012925222No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA27760.161703619866012No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG27720.16147061753191114No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG27370.1594318471085284No Hit
GAAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCT27190.15838333660507445No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC27120.1579755825203979No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACTAG26470.15418929459125857No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG25690.14964574907629136No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT25680.14958749849276615No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT25640.14935449615866525No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC25020.14574295998010162No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG24410.14218967438506314No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA24410.14218967438506314No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT24290.1414906673827605No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA24050.1400926533781552No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT23860.13898589229117603No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC23270.13554910786318802No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA23110.1346170985267845No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC22910.13345208685628007No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22610.13170456935052346No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTAGGC22600.13164631876699825No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA22560.13141331643289736No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC21840.12721927441908149No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG21780.12686977091793017No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT21750.1266950191673545No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA21660.1261707639156275No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT21260.1238407405746187No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT21220.12360773824051782No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT20850.12145246665008468No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT20840.12139421606655945No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC20830.12133596548303423No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG20690.12052045731368113No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC20530.1195884479772776No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAAGGTGTGCAGAACCTTACT20530.1195884479772776No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA20410.11888944097497497No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG20140.11731667521979403No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA19910.11597691179871396No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA19670.11457889779410867No Hit
GTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTAGAAAACTACT19650.11446239662705822No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG19360.11277312970482682No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC19320.11254012737072594No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC19300.11242362620367549No Hit
GTATAGAGAGGAATCAAGACTAGAAAGGCAGAAAATAGAGGGGGTTAAGC19110.1113168651166963No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC18950.11038485578029276No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18950.11038485578029276No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC18860.1098606005285658No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA18820.10962759819446491No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATAGC18640.10857908769101095No Hit
AAACAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA18510.10782183010518308No Hit
GGAGTACCCTGTCTGATCCAGCGTCATTTGTTTTGCTCCAAAGAGTTTGG18280.10648206668410301No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC17320.10089001066568185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA25150.0110.124972
GGTACTG26350.0104.834635
CAGGTAC27100.0101.9333043
GTACTGA30000.092.8129046
AGTAGAA17100.080.559711
CGCAATT451.233313E-680.55502145
AGGTACT35950.079.264
CACGAGC3950.077.0881145
ACTGATC41950.071.728088
AGCAGGT39100.070.649431
TACTGAT42300.066.5065467
GCGAAAG3800.064.871772
CGAAAGC3850.064.029283
GACAGTA22350.056.447517
GGTCAAA3600.056.39181
CTCCGAC1301.8189894E-1255.768864145
TAGAAAC24650.055.2969743
AAATATA4050.053.706475
AAGCGCC553.219759E-452.726925145
TCTCCGC4300.052.267094145
Produced by FastQC (version 0.11.5)