FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083633
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG900.096.6655145
GCGAAAG8750.039.781452
CGAAAGC8700.039.1765373
TATACCG4500.038.667139
AGCGAAA9700.034.390111
TATAATA16000.033.086462
CCGATCA1601.8782339E-631.719139
TCTCCGC8050.030.620749145
GTGATCG1504.400609E-529.0003459
ACGTTCG1050.007700863827.6260095
GTATATA12250.026.6393621
ACTTACG1409.983549E-425.899382
GTATAAT10200.024.8837181
ACCGTGT6750.024.7039998
ACCGTTC2651.3776662E-724.6229368
TATAGCG1550.001806494123.392995
GGCCGAT1550.001809018323.3873777
TAGACAG17150.021.9880455
CACGAGC4951.8189894E-1221.969433145
GTATAAG5750.021.4401821
Produced by FastQC (version 0.11.5)