FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533840
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4100.051.291542
AGCGAAA4950.042.4825131
CGAAAGC6500.033.4687463
ATATACG1551.4632187E-632.748994
CACGAGC7450.031.140541145
ATGCCGG3300.030.757181145
TTACGGA1902.0739026E-730.5328924
CCCACGC8200.026.524052145
GCACCGT4650.024.9524276
CCGTACA2651.3744648E-724.6279452
TACGGAG2951.4259058E-824.5815665
TCTCCGC9250.024.296986145
GTCCTAT15350.024.0923521
AACGTGC3052.0345396E-823.7725128
TGTAGCG4103.8198777E-1122.9927582
GCCGTCG2252.6529982E-522.555265145
TCTCGTT3253.993955E-822.307407145
CGTCTTG3655.691618E-921.849037145
GTATAAT10550.021.307211
GGCGCGT2053.666375E-421.2206239
Produced by FastQC (version 0.11.5)