FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153984
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC1206.002665E-1154.389221
TCGTACG450.00963951748.334759
CCGTACA2250.038.6767772
GCGAAAG11600.031.8833372
AGCGAAA11550.031.393491
ATCGCGG1402.7484573E-531.070896145
CGAAAGC12850.028.7818433
CGTACAC3750.027.0737443
GTATAGA16950.023.1033841
GTCCTAT15150.022.9763031
CTCCGAC4505.456968E-1222.555168145
CTACGAC1952.6114786E-422.3135243
TAGACAG19500.021.197855
TACACCG6300.020.7197025
CACGAGC12250.020.71393145
TATAGAG11050.020.3446852
TACGACC2155.049831E-420.2378484
GTATAGT6450.020.2378481
TAGTACT8350.019.975284
TATACTG9800.019.2397255
Produced by FastQC (version 0.11.5)