FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560645
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15720.10072758378747249No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG553.2176866E-452.7334945
GCGAAAG5800.045.0038642
AGCGAAA6350.041.1058961
GTGATCG1553.471905E-837.4201749
CGAAAGC7400.037.2329333
TATTACG1701.03105325E-425.5904352
TTACGCA2859.895302E-925.4407844
GCAATCG4950.024.898737145
TACGCAG2802.3416942E-723.3063225
GTATACC2202.228359E-523.0701664
TCTCGTT4800.022.65602145
GTATTAG3604.8476068E-922.1546841
CTCCGAC2953.8776307E-722.11842145
TATACAG7150.021.296225
TAGTACC9250.021.163984
AGTGATC15900.020.9753028
GTATTAT14050.020.6423441
ATGCCGG3208.477782E-720.390417145
TATACTG6150.020.0430165
GCCGTAG1850.005045611419.596281
Produced by FastQC (version 0.11.5)