FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197542
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9200.062.2688871
GCGAAAG9550.059.2274482
CGAAAGC14100.040.629343
GTATACG4101.8189894E-1224.7614441
TGATCCG3902.0008883E-1124.1674869
CGCGCCA3902.0008883E-1124.1674869
ACGCGCC3102.4203473E-823.387898
AGACGCG2502.2770073E-623.2050116
CTAGGAC12150.022.6797943
GTATATA13250.022.4388071
CTATGCG2653.7762475E-621.891524
TATAAGG11600.021.8797252
TATACTG9700.021.6799375
ACTCCGC3851.0524673E-820.7149898
CCTATAC3951.4106263E-820.1942353
CTATACT10650.019.7460484
TATAGGC8550.018.6592
GACGCGC3151.685192E-518.4124837
ATATAAC10350.018.2164943
ATACGTG7650.018.0104265
Produced by FastQC (version 0.11.5)