FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520573
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6400.057.7843671
GCGAAAG6600.057.132022
CGAAAGC7600.048.660523
CACGAGC8050.035.123936145
ACCGTTC1352.1430504E-532.221968
TATACTG9500.030.5320915
TTATACT6900.026.2730854
GTGATCG2101.5558593E-524.1664689
TAATAGT7600.020.9908124
GTATAGG4201.2660166E-920.7182031
ATTATAC6100.020.208743
GTAGTAC3401.5131354E-619.1948071
TAGTACC6600.018.6777744
TCTCCGC10100.018.663214145
CGCCGCA1950.006834692318.5932582
ATGCCGG3953.1255877E-718.354282145
GTCCTAT9600.018.128431
GTACTAG4959.744326E-917.5790821
TATTATA11700.017.353712
GTTATAG3352.8551647E-517.3167081
Produced by FastQC (version 0.11.5)