FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525633
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC30650.20090021650029857No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC21520.14105620421162887No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT21130.13849988824310958No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC19740.1293889159450536No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA19520.12794689155255556No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC18640.12217879398256332No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC17820.11680397579234324No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT17730.11621405672268494No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT15420.10107280060145526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4700.057.0922131
GCGAAAG5350.050.1557772
CTCCGAC2250.045.110348145
TCGACGG3400.042.646336145
TCTCCGC9100.040.63118145
ACCGTGT10500.038.667288
CGAAAGC6950.038.6091233
AGCACCG10200.037.6845175
CCACGAC3550.036.759937145
CCCACGC4950.036.61554145
GCACCGT11150.035.774626
TGATCCG1051.8539991E-434.5243579
CACCGTG13050.033.3349537
GTCCTAT7200.031.2249811
CCGTGTT14150.028.6930339
TGACGAG1306.472748E-427.8932861
CGTAGAC1050.007698013427.6276343
ATAGCAC18450.026.33613
GTACCGT1751.2535007E-424.8648683
TCTCGTT3551.546141E-1024.506624145