FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493971
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16220.1085697111925198No Hit
GTGTATAAACAGTGTGTTGCCACATTTAATTTGGGAGGGGGCGTTTAAAA15340.10267936927825239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4150.068.149572
CGAAAGC5650.048.773263
AGCGAAA5950.046.3125461
GAGCGAA1451.9252184E-840.0086631
CAATCCG750.001483018638.669869
CGTAAGT750.001483262638.6685687
CCCACGC6000.032.62442145
CACGAGC4950.032.22165145
TCTATAC13750.031.1168693
GTGATCG1902.0762491E-730.5288419
GTGTATA13900.027.1281741
TGTATAA13600.026.1278552
TATACAT5850.026.0330225
CTATACC15600.025.567294
GTTCTAT15650.025.484751
TCGCGTT2101.555951E-524.166237145
TATACCA17600.023.4867695
TATAGAC3102.4134351E-823.3929442
GTATACA5900.023.3525121
CTCCGAC2202.2300654E-523.06777145
Produced by FastQC (version 0.11.5)