FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905536
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC8900.046.4405823
AGCGAAA8900.045.6258351
GCGAAAG9100.045.4199142
TTATACG1051.8524684E-434.5297554
TGATCCG1902.0775224E-730.527579
ACGCGCC3650.029.7957468
CGCGCCA3850.028.2479159
AACGCGC3057.1122486E-1026.1479287
TATACTG11100.025.4780315
TCTCCGC4850.025.41208145
CACGAGC5200.025.095867145
TCCTATG15050.022.6450962
GCGATCG4006.91216E-1021.74975145
TGAGCGC6700.020.559467145
ATACCGC5403.6379788E-1220.1428876
AAACGCG4001.6301783E-819.9414586
TAGTACT8000.019.9409374
ATATAGC4553.430614E-919.1241743
TAGTACC12900.019.111824
ACCGTAC1950.00684115318.5905088
Produced by FastQC (version 0.11.5)