FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760647
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7400.040.179861
GCGAAAG8450.035.187092
CGAAAGC9800.030.3398913
CGAGGCG4051.8189894E-1225.061434145
GTATATA7950.024.6293471
AGACCGC4201.8189894E-1224.166382145
CTATGCG2751.965218E-723.7337344
AGAACCG2451.908933E-623.6799165
GTATAGG4303.6379788E-1223.6110781
TACGCCG2552.7026272E-622.7512935
ATGCCGG4800.022.655985145
TATGCGG3253.9828592E-822.3137665
GAACCGA3809.025825E-920.9925586
CACGAGC5700.020.350637145
TACTGCG2608.082495E-519.5190017
TCCGACT4303.7340214E-818.5515615
TACACGT2359.1870443E-418.5156785
TATACAT10050.018.0397385
GTATAAG5301.1768861E-917.787861
CTTCGAC2050.00911478117.6877423
Produced by FastQC (version 0.11.5)