FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480642
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7800.055.7734831
GACGCGC5050.054.5553177
CGCGCCA9050.050.4704139
ACGCGCC9100.050.1931048
GCGAAAG8700.050.003822
AACGCGC4100.044.2080157
AGACGCG7450.036.9834376
CGAAAGC12900.034.2855643
AAACGCG5300.034.2014126
TAAGACG9200.032.3128974
AAGACGC9050.032.047295
CAAACGC6250.029.00285
CACGAGC5300.028.72629145
TCTCCGC11900.027.415848145
GGGTTAC13000.026.7712751
TGATCCG4100.026.5248099
GTCCTAT18850.025.3865531
TAGACCG1500.001494056324.1689995
GCAAACG7750.023.3893534
GCGCTAT4256.184564E-1122.1781641
Produced by FastQC (version 0.11.5)