FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2242_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2242_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632215
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22810.13974874633550113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA5750.045.3907179
GCGAAAG5850.044.6271132
ACGCGCC6300.042.5788158
AACGCGC3600.034.235677
AGCGAAA7700.032.9632071
CACGAGC7600.031.479856145
GACGCGC5150.030.9704747
GAATAAC9600.030.2162741
AAACGCG3850.030.137796
CGAAAGC8500.029.8607883
AGACGCG6350.027.401222145
TAGTGCG2405.2625182E-827.1946475
CTCCGAC2601.1458178E-725.09583145
TAAGACG6950.025.0425534
ATAAGAC7950.023.7169253
GTCCTAT12900.023.6108571
TAATCCG1550.001806212423.3932465
AAGACGC6250.023.2060995
TCTCCGC10000.023.199701145
TGATCCG3452.9685907E-923.1156439
Produced by FastQC (version 0.11.5)