FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT33290.16936321669028967No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23940.12179499572140386No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT22470.11431635563324749No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22130.11258660214346983No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT20460.10409046000250306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCCG951.9218933E-645.7892849
CACGAGC5500.044.817993145
GTTGACA16750.037.6603931
TCTCCGC4750.035.105114145
GTCTACG1051.8533028E-434.527181
ACATGGT19900.031.6990745
GTATTAT28400.031.1474041
TCGGATT950.004728491430.526188145
ATGCCGG4600.029.945526145
CCGACTA4300.028.6655864
ATACCGC2054.0390478E-728.295446
TATTGGG20350.027.7914092
GGTCCTA28550.027.4254559
GTAATGC7550.026.890041
GTTACGT2259.1072616E-725.7802931
TTACGTG2259.1072616E-725.7802932
GCGAAAG3950.025.6987081
ATGGTCC30850.025.3807687
AATACCG1450.001226167525.0024385
CATGGTC25550.024.9730826
Produced by FastQC (version 0.11.5)