FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587062
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22590.14233848457086112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC2250.041.88849145
AGCGAAA4900.038.4750821
CGAAGCG800.00203518136.2565085
GCGAAAG5300.035.5724222
TCTCGTT5550.035.26993145
CCGACTA1902.0746302E-730.5317964
CGAAAGC6350.029.6903673
GAATAAC10950.028.4745331
TATATCG1050.007703042627.6240065
TACTGCG1050.007703042627.6240065
ATGCCGG4600.026.793222145
ATACCGC1958.746523E-626.0303136
TAAGTCG1409.986719E-425.8975055
GTATATA6850.023.2881161
TCTCCGC6300.023.015652145
GTCCTAT18900.021.8683151
GGTTATA7300.021.852551
GTTGACA12000.021.753221
GTATTAT15350.021.7295991
ACGCTAA3355.495349E-821.6449951
Produced by FastQC (version 0.11.5)