FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951320
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8200.053.9447171
GCGAAAG9150.049.136422
CGAAAGC10750.042.4987533
ACGCGCC3700.031.352588
CGCGCCA3350.030.2996969
ATGCCGG3001.8189894E-1128.999651145
TGATCCG5200.027.885719
GACGCGC3700.027.4335087
CGTTACA3253.9855877E-822.3125691
TCTCCGC6200.022.217474145
GTGATCG3055.343518E-721.3942839
ATACCGC8250.021.0960626
TACGCCG1750.003654784820.7193455
ATATACC8550.020.3558484
AAGGTCG2505.9670023E-520.3049585
TACACTG14850.020.0217235
AGCACCG8750.019.8905725
GTGATCC14300.019.7735028
AGACGCG5204.3655746E-1119.5246
TACCGCC10450.019.4266017
Produced by FastQC (version 0.11.5)