FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373646
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG18710.1362068538764718No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT17990.13096532876738257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6000.056.813162
AGCGAAA6400.052.12721
CGAAAGC6850.049.763353
GACGGAT3000.036.253167
CCTTGTA3900.035.3338852
ACGCGCC5350.032.52628
TAGTACT2705.456968E-1232.234424
GCGAATG3800.030.52564145
CGCGCCA5400.029.5396129
TATAGTA2553.0140654E-928.4421332
TGTACTG8450.028.3253575
GTATTAA14200.028.0905911
TGATCCG4450.027.6990439
CTTGTAC6050.027.5724143
TAAGACG6400.027.1977924
GACGCGC6250.026.6823257
CACGAGC6000.026.582747145
TCTCCGC8000.025.374441145
GTACTGT11250.025.1446766
CAGGACG7050.023.6614344
Produced by FastQC (version 0.11.5)