FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019097
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA14110.13845590753382653No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12240.12010632942693386No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11980.11755505118747284No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10820.1061724251960314No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10650.10450428173176841No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10440.102443633922973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGCG2300.053.6020856
GTTACGT2000.050.7643241
ACTACGC752.546365E-548.3469771
TTACGTG2100.048.3469772
AACGCGC450.00963965248.3327457
GACGCGC752.5508016E-548.3327457
ACGCGCC1203.621608E-948.3327458
CGCGCCA1351.0260919E-842.9624379
GTTGACA9600.041.5481831
GTCGAAG1451.9232175E-840.011293
AGTCGAA1451.9232175E-840.011292
ACATGGT11100.037.240245
ACGTGTT2850.035.624094
CCGACTA4350.035.0098844
TACGTGT2950.034.4164923
ACGGCGT850.00274217434.11723145
CAGTGCA8250.033.4033662
CTACGCA1102.4321482E-432.963852
AAGTCGA1801.2892451E-732.231321
ACGAACC4850.031.4006145
Produced by FastQC (version 0.11.5)