FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655100
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17460.1054921152800435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2050.070.737851
TTACGTG2900.050.0043452
ATGCCGG2350.040.10623145
TAGTCGG2700.037.5924538
GTTATCG800.00203613136.253153
ACGCGCC1251.2656197E-534.799878
ACGTGTT4200.034.5268064
CTCCGAC2103.783498E-1034.52368145
TATACTG7200.034.2390865
GTTGACA15400.032.4865881
TCTCCGC6500.031.230654145
TACGTGT4650.031.1855073
GCGAAAG5300.030.0969541
CGCGCCA1453.491853E-529.9998869
CGAGGTG5900.029.494095
ACATGGT15600.029.2813915
ACTCCGA5850.028.506751
CACGAGC3850.028.246649145
AGCGAAA4650.028.0669561
CTCGCAC1555.499313E-528.0669543
Produced by FastQC (version 0.11.5)