FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997385
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6850.041.2870332
CGAAAGC8000.035.352023
AGCGAAA8100.034.9155771
TATACTG13050.027.2285925
TCTCCGC7700.026.363205145
CACGAGC3706.661139E-921.5537145
ATACCGT1700.003088769521.3295866
TTATACT13300.021.2643744
ATGCCGG3259.841333E-720.076593145
TAGTACC11400.019.7195264
GCACCGT7650.018.9596336
GTATACT6550.018.8211984
ACTGATC14200.018.381828
GGTACTG9100.018.3284635
GTACTAA7550.018.2493021
TATAGAC6553.6379788E-1217.7140672
ATTATAC8250.017.579873
CTCCGAC2901.8667814E-417.499712145
ACTCGCG2901.8667814E-417.499712145
GTATAAT8750.017.404071
Produced by FastQC (version 0.11.5)