FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468742
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5050.034.4597741
GATAGCG3600.034.23575145
TCTCCGC4550.031.867798145
CGAAAGC5700.031.802243
GCGAAAG5350.031.172142
AACGTAG1504.395306E-529.0046315
CCGAGGT5300.028.7300244
GTCGTCG1050.00771000727.618753145
TCCGAGG6100.026.1508263
AAGGCGC3801.2732926E-1124.80237145
TATACTG9400.024.6847935
CGAGGTG6200.024.5603715
ACTCCGA5650.024.3835931
GTATAGG3052.0336302E-823.773481
CCGACTA3304.6875357E-821.9724584
ACGTAGC1650.002601593721.969467145
ACATGGT8950.021.8750025
CACGAGC3406.4374035E-821.323303145
ACTGATC8950.021.0626648
GTTGACA9750.020.823131
Produced by FastQC (version 0.11.5)