FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182676
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26060.11939472464076208No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC24150.11064399846793568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6650.032.7139131
GCGAAAG7200.030.2149312
GTATTAT23600.029.1906951
GTGATCG3550.028.5918989
GTTGACA14100.028.2863161
TACTGCG2654.5547495E-927.3644645
CGAAAGC8250.027.2483753
GAATAAC19000.025.5713731
TCTCCGC6900.025.217121145
ATGCGCC2601.1461634E-725.095884145
GCGTTAA2951.4297257E-824.5765699
GTTACGT1550.001806874823.3922041
ACGTAGG1550.001806874823.3922041
ACATGGT17750.022.4696945
GTATATA10750.022.2606741
GGTCGTT2951.3343742E-722.119427
TATACTG14550.021.4307985
CACGAGC4801.4551915E-1121.145607145
GTACTGC7350.020.718814
CGTCTTG5301.8189894E-1220.518646145
Produced by FastQC (version 0.11.5)