FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566890
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC25300.1614663441594496No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16560.10568706163163975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG850.002739579434.1249541
GCGAAAG3900.033.4687082
GTATACG1102.4337145E-432.9616051
GTATTAT15850.032.9408071
CGAAAGC4550.030.2812123
GCGTTAA1453.4918656E-529.9997229
ATGCCGG2003.2565913E-728.999731145
GCACCGT6450.028.1067946
AAGGTCG2853.237801E-1027.9821979
AGCGAAA4800.027.1933211
GTATAAT6150.027.1196291
GTTGACA10900.026.6112021
CTCCGAC1658.42209E-526.363392145
GATATGT8200.024.76141
AGCACCG6750.024.7089965
ACATGGT11900.024.3749685
TATTATT21300.024.1718432
ACCGTGT7600.023.8484638
TATATAG7950.023.715773
GTATATA7600.022.899641
Produced by FastQC (version 0.11.5)