FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078769
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA2950.036.86392145
GCGAAAG7150.036.5074042
AGCGAAA8450.029.1740191
TGAGCGC7750.028.064142145
TCTCCGC8050.027.018276145
TATACGA1358.080611E-426.8547232
CGAAAGC9250.026.6515033
CCATCGA5500.025.0481369
GATAGCG2151.8671852E-523.604338145
CCGTACA1851.8305623E-423.5154651
TCGCCAT4750.022.894432145
GCGTTAA1600.002174885722.6581291
ACATCGT3905.020411E-1022.311696
ATGCCGG5250.022.094944145
CACGAGC6400.021.523151145
TACATCG3757.761628E-921.2699645
GGACCGT3106.2398976E-721.0521586
CTCCGAC3457.5153366E-821.014214145
ATCGTCG3208.469069E-720.3932978
GTATAAT7950.020.0645561
Produced by FastQC (version 0.11.5)