FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2241_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2241_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563485
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1056.9016096E-848.3407445
AGCGAAA5500.035.5963671
GCGAAAG5650.034.651332
GCGGTAT2750.034.277986
TATAGCG2800.033.665882
ATCGTCG4200.031.0712248
CGAAAGC6450.030.353493
CTATACG2003.251953E-729.0044461
ATAGCGG3301.8189894E-1228.5649853
AGCGGTA3351.8189894E-1228.1386415
CGGTATC3351.8189894E-1228.1341427
TCGTCGA4700.027.7657749
GTATTAG3555.456968E-1226.5533661
ATGCCGG3306.184564E-1126.363462145
TAGCGAG2259.1008405E-725.781736
TATATAC8350.025.1835023
AGACCGC6200.024.55629145
CTCCGAC2751.9702202E-723.727116145
TAATACA12750.023.31734
ACATCGT5600.023.3071466
Produced by FastQC (version 0.11.5)