FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2240_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2240_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480168
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19280.13025548451256885No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC19100.12903940633765895No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17880.12079709870771425No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT17490.1181622626620762No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC17320.11701374438577243No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA16630.11235211138195124No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT16560.1118791920917085No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT15870.10721755908788733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2350.064.7907941
TTACGTG2450.062.146282
CACGCTA450.009634091548.340899
ACGTGTT3300.046.13894
GACGACT1053.823923E-641.430854
TATACTG7700.040.4919745
GTCGTCG800.002037056336.249546145
GTTGACA14800.035.7621041
GCGATTA1051.8542819E-434.523376145
GATATAC3600.034.2379953
TACGTGT4450.034.2153663
GCTACTA2551.8189894E-1234.1229829
AGCGAAA4700.033.9380381
GATAGCG4750.033.57852145
TAGTCGG1956.9867383E-933.4667668
GCGAAAG5250.033.144682
CGAAGCG1102.4344766E-432.9596989
CGTGTTG4650.032.745955
ACATGGT16000.032.6289985
CATTCTA16250.032.1248442
Produced by FastQC (version 0.11.5)