FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2240_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2240_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423257
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC15420.1083430469690295No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC15120.10623520558830907No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15070.10588389869152233No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG350.003563983462.1553085
GTTACGT2700.059.085911
TTACGTG2900.055.011022
TACGCCT400.00604661454.38594
AAGGTCG1600.054.3744359
AGCGAAA2650.049.2551541
ACGTGTT3850.043.320374
GATAGCG4600.039.401764145
TACGTGT4400.037.9053233
GCGAAAG4100.037.1415862
CAAGCGG800.002034901436.2572673
CACGAGC3150.034.52345145
CGTGTTG5100.034.1244855
TATACTG8000.033.5379685
CGAAAGC4550.033.4682433
GTATATC2506.91216E-1131.9063951
GTTGACA11300.031.4443551
ACATGGT11750.030.8572445
GATATAC2601.10958354E-1030.6792263
GTTATGT8650.030.1794571
Produced by FastQC (version 0.11.5)