FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2240_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2240_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756425
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG81930.2972328287546369No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG77680.28181430657463924No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG60730.2203216122332369No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT60250.2185802261987901No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT57230.20762400573206236No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT55800.20243612650443962No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC53980.19583337112382887No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA52980.1922054835520647No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA51980.18857759598030058No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA47820.1734855836817617No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC46520.16876932983846832No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA46150.16742701143691557No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT44850.16271075759362216No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA44380.161005650434893No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG43000.1559991655858585No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT42470.1540763851728235No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT41150.14928757357809483No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC39840.14453504085908378No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT38620.1401090180215315No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG37950.13767833334844953No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG37370.13557415855682634No Hit
GAAGAAAGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACATCT35100.1273388537689217No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA33850.12280399430421651No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT33430.12128028152407558No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT32600.11826913483951132No Hit
ATTTCATCTCTCTACTGGTCATTAGTCTGTTCAGATGTCTTTTTCTTTAG32380.11747099957372321No Hit
GGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT31960.11594728679358228No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC31870.11562077691212351No Hit
GTCTGTTTCTTTAGAATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG31730.11511287265207652No Hit
AGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATCCAAAAGA31500.11427845851057075No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA31380.11384311200195907No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA31290.11351660212050027No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA31240.11333520774191208No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA31130.11293614010901801No Hit
CAGATTCTCAGCGAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGA30890.11206544709179464No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA30080.10912685815866567No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30020.10890918490435982No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT29360.10651477910699547No Hit
GATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGATGTTCCCTGA29160.10578920159264263No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA28980.10513618182972509No Hit
GTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGA28780.10441060431537227No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT28550.10357619017386652No Hit
GTTCATATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTC28090.101907361890855No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA27600.10012969698069056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA502.006068E-458.028044
AGTCCGC2400.057.394417145
CTCCGAC1900.057.23543145
ACATGGT30800.056.049825
CACGAGC7850.055.41265145
GTTGACA31500.055.0345341
GGTTATA9650.050.361131
GTTACGT2500.049.3238371
TGCGTTA4200.046.606848
TTACGTG2650.046.5319182
GCGTTAA4250.046.059369
ATCTATG17150.045.678051
GTATTAT34700.044.9424671
ACTGGTT13250.044.3421865
TCTCCGC7950.042.86058145
GGTCCTA50600.041.9817129
CATGGTC41600.041.847156
CAGTGCA20350.041.3467642
GTTATGT9550.041.0145871
GTTATAT12050.040.3306922
Produced by FastQC (version 0.11.5)