FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2240_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2240_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608011
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC30800.19154097826445218No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20820.12947672621642514No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG20350.12655386063901305No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC19470.1210812612600287No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG17820.11082013742443304No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA17730.11026043976067328No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT17250.10727538555395455No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16140.10037244770091747No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA16110.10018588181299755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1402.728484E-1046.61274
AAGGTCG1903.6379788E-1241.9734739
TGCGTTA1953.6379788E-1240.8972248
GCGTTAA2005.456968E-1239.8747949
CGACAGC750.001482785738.6712766
ATGCCGG5900.036.864216145
GTATTAT12250.036.6982541
GGTTATA4600.036.2543221
CAGTGCA8500.034.1217162
TCTCGTT4900.034.030437145
ACATGGT16600.033.6335265
GTTGACA16850.033.5648351
TCTCCGC7650.033.16976145
GTTACGT1551.4643774E-632.7458421
TACTGCG2455.4569682E-1132.55495
TATACTG10300.032.38255
GTAGAAA7600.031.4840162
AGACGAC2101.4393663E-831.0751343
CGAGGTG4750.030.5299575
GTACTGC6150.029.475064
Produced by FastQC (version 0.11.5)