FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683660
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT26590.15792974828647113No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19200.11403727593457111No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT18790.11160210493805162No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT17360.10310870365750804No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16900.10037656058824228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3150.062.163651
TTACGTG3400.057.5945052
CGTCCGA400.00605070454.3770378
CCGACTA4700.044.750344
TACGTGT4850.038.880143
ACGTGTT5150.036.6152764
TCTCCGC5300.035.565254145
CACGAGC3150.034.523052145
CGCTAAT850.002737622534.1300776
GGTGCGA1501.1341708E-633.83468
TATACTG7750.032.7538645
TGAGCGC7300.031.780125145
TATATAG6650.031.628063
CCACCGA6850.030.7037011
GTGCGAT2151.8118953E-830.3490729
CACCGAC7150.029.4163062
AAGCGGC1504.3895554E-529.0105634
GTTGACA13550.028.9026571
GACGACT2054.0290615E-728.302994
CGTGTTG7200.028.2047165
Produced by FastQC (version 0.11.5)