FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051967
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13210.12557428132251297No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11040.10494625781987457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC1650.057.120163145
GTTACGT1203.610694E-948.3485341
CTCCGAC901.3238096E-648.332447145
ATGCCGG1453.8562575E-1044.999176145
ATCTATG5200.034.866731
TCTCCGC4200.032.797016145
CGAGGCG2257.930794E-1032.221634145
GCGAAAG3450.031.531652
GTTGACA10600.031.4721571
AGCGAAA2859.094947E-1230.5359131
GTTATGT6200.030.4127861
TTATTCG1204.058625E-430.207779145
GTACCGC1453.481951E-530.0108626
ACATGGT11600.028.7590435
CTTATAT4600.028.3784871
TTACGTG2054.0283885E-728.301582
GGTGCGA1555.4995973E-528.0653348
CAAGCGG1306.471268E-427.8933833
GTGATCG1050.007707533427.6198549
CGAAAGC4000.027.196053
Produced by FastQC (version 0.11.5)