FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263524
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG23090.10200907964748772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6550.052.0326422
CGAAAGC8400.041.436333
GTGATCG4150.036.6879439
ACGCGAT1209.565607E-636.251988
AGCGAAA11600.029.3798521
GTATGGA17550.028.0958061
CGAGACG1050.00771091127.618729145
GGTACTG12800.025.4930555
TATATAC10600.025.3113423
GTATACT4900.025.1577724
TATACTG14000.023.825895
GACAGTA13100.023.7984857
AGGTACT13500.023.6340524
TATACAT16000.023.5669125
ACCGTAC1902.1919052E-422.898521
GGATAGC16900.022.7409465
GTATAGG5200.022.3113771
ACTGATC16250.022.3089128
TTTGGAC15800.021.5704943
CGCGATC2104.3155038E-420.714969
Produced by FastQC (version 0.11.5)