FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814822
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG30580.16850137368843887No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC21930.12083829708919111No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT19090.10518937945429359No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG18420.10149755733620157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6050.050.3372232
AGCGAAA7100.042.8929861
GGATAGC14050.041.8026775
AGGTACT9450.037.5975574
CGAAAGC8300.037.5652053
GATAGCA16400.036.2547876
GGTACTG11700.033.4659585
GTATGGA19600.032.555321
GTGATCG4850.031.3915739
CAGGTAC10400.029.979923
GCAGGTA9950.029.8783172
ACTGATC13450.029.6466718
TGTGCGC2603.7252903E-927.8844579
ATTAACG1050.0077051127.6226963
TACGCCA1050.0077051127.6226964
TGGATAG21500.027.3175624
TTTGGAC13900.027.1258853
TATGGAT23550.026.479082
ATAGCAA22900.026.2771437
CGTTTAT1701.0308206E-425.5916161
Produced by FastQC (version 0.11.5)