FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2239_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2239_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865219
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA187601.005780018324926No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC163690.8775913176951339No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG157810.8460668693595765No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT128630.6896241138440045No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATT115340.6183724270447599No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA114020.6112955100714715No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT101860.5461020931054209No Hit
TTTCTTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAG94800.5082513098998026No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG88580.47490401931354975No Hit
GGATACACCATGGACACAGTCAACAGAACACAAAGGGGAATTCTTGAGGA88020.47190169090063955No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA67290.36076192661558776No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG67180.36017218353448044No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGA62610.33567103916483804No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT58190.31197408990579656No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG57560.3085964704412726No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC57400.3077386623232982No Hit
GGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGA56750.3042538168440274No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA55670.29846361204770055No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA53390.2862398463665661No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC50110.2686547799480919No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG49680.26634942063103584No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG47800.2562701752448372No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG45300.24286692340148797No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA42460.22764082930744325No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG42380.2272119252484561No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG38820.20812569462352679No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA38720.2075895645497928No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT37270.19981567848065024No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG37160.1992259353995429No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA35830.19209540541888112No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC35610.19091591925666637No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT35070.18802081685850294No Hit
CAGCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAA34830.18673410468154142No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA34310.1839462282981248No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT32710.17536814711838128No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT32660.17510008208151429No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC32620.1748856300520207No Hit
GGTTTAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA32510.17429588697091333No Hit
ATATTATTCACTTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC31850.17075742848426914No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA31500.16888097322620024No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC30740.1648063846658221No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC30570.16389496354047434No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG30510.16357328549623396No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG30510.16357328549623396No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC30460.163305220459367No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA28970.15531688236073085No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC28730.1540301701837693No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGCGAATCA28270.15156397184459303No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC28170.15102784177085907No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG28110.15070616372661869No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT28090.15059893771187188No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT28000.15011642064551134No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG27850.1493122255349104No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG27480.1473285442620947No Hit
CGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCC27390.1468460271957341No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA26950.14448705487130467No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT26840.1438973117901973No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG26360.14132388743627425No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG26130.1400907882666861No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC25960.13917936714133836No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG25870.13869685007497778No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA25520.13682039481690889No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT25440.13639149075792173No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC25430.13633787775054834No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA25270.13548006963257397No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA25160.1348903265514666No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTGCCAGCGCAAAAT25150.1348367135440932No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25140.1347831005367198No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA25050.13430058347035925No Hit
CCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGC25010.13408613144086565No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG24500.1313518680648224No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG24460.13113741603532883No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC24420.13092296400583522No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCT24260.13006515588786088No Hit
GAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGA24140.12942179979938012No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA24030.12883205671827275No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT23800.12759895754868464No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG23790.12754534454131125No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT23200.12438217710628083No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA22870.12261294786295872No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA22780.12213043079659813No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA22670.12154068771549077No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT22470.12046842756802284No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG22370.11993229749428888No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA22260.1193425544131815No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA22230.11918171539106132No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC21720.11644745201501808No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT21220.11376680164634824No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT20780.11140782932191878No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT20420.10947776105647648No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC19780.10604652858457908No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT19760.10593930256983229No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG19760.10593930256983229No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA19740.10583207655508549No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTG19620.10518872046660471No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG19580.10497426843711115No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC19540.10475981640761754No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT19040.1020791660389477No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC18990.10181110100208073No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA18940.10154303596521375No Hit
ATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCT18910.10138219694309356No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG18900.10132858393572014No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT18810.10084606686935958No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC100.007091566144.99854145
ACGCGTT100.007091566144.99854145
AGCGTGC151.2302247E-4144.99852145
GCAGGTA22350.0111.611162
CGTCTGA203.8634456E-4108.7722241
TACGAAC203.8634456E-4108.7722243
GGTACTG23100.0107.9903265
GACTTAA553.6379788E-12105.45346145
CAGGTAC24550.0101.904733
AGTAGAA25600.0100.5576551
CTCCGAC650.0100.38359145
CCCACGC800.099.68649145
AAAGTGC29600.096.0151148
TCTTAAA30300.095.250493
GTACTGA26700.093.701436
AAATATA3500.087.020115
GATACGA259.380414E-487.017781
CTTAAAA33450.085.630354
AAGTGCC33200.084.94879
TAAAAGT35000.081.840356
Produced by FastQC (version 0.11.5)